- Organ transplant
- Identity testing
- Zygosity testing
This test is based on genotyping of 16 loci (15 polymorphic STRs and the sex-specific Amelogenin marker): Penta E, D18S51, D21S11, TH01, D3S1358, FGA, TPOX, D8S1179, vWA, Amelogenin, Penta D, CSF1PO, D16S539, D7S820, D13S317 and D5S818.
This test is performed using the PowerPlex 16 System (Promega). Markers are genotyped by multiplex PCR amplification and capillary electrophoresis.
Analysis is dependent on correct reporting of family relationships. DNA variants located in primer binding sites and rare genotyping errors can result in inaccurate diagnosis. In addition, DNA obtained from formalin-fixed paraffin-embedded (FFPE) samples could lead to genotyping errors due to DNA degradation and preferential amplification of certain alleles (allelic drop-out).
Turnaround time: 3-5 working days
Specimen accepted:
- 1 x 4 mL blood in EDTA tube (purple top tube) – 2 ml for newborn
- Amniotic fluid: 2 x 1.0 ml EPPENDORF
- Amniotic fluid: 20 ml (for possible further testing)
- Fetal cord blood: 500 ul in EDTA tube (purple top tube)
- FFPE specimen : 10 x 5 um scrolls in 1.5 ml tube or 6 x 5 um unstained slides
- For any other sample type, contact the laboratory for testing availability
Submit your test request using our molecular genetics requisition.