Bernard Turcotte, PhD

Primary Axis: 
Endocrinology, Diabetes, Nutrition and Kidney Diseases
Email: 
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Research Focus: 

Research in my laboratory relates to functional genomics in yeast, focusing on the Gal4 family of transcriptional regulators which includes over 50 members. The function of many of these Gal4 members is unknown or poorly understood. To better understand the role of these transcriptional regulators and identify their target genes, we are using various approaches including genetics, molecular biology, and whole-genome analysis of gene expression with DNA microarrays. We are also determining the role of related Gal4 members in conferring resistance to antifungals in the pathogenic species Candida albicans. Another field of study focuses on the mechanism of action of the anticancer drug tirapazamine which is currently under clinical trial.Yeast, drug resistance, azole, transcriptional regulator, DNA binding protein, Gal4, zinc finger proteins, Candida albicans, genome-wide analysis, DNA microarray, gene expression, genome-wide localization of transcription factors, phenotypic analysis

Keywords: 
Yeast, drug resistance, azole, transcriptional regulator, DNA binding protein, Gal4, zinc finger proteins, Candida albicans, genome-wide analysis, DNA microarray, gene expression, genome-wide localization of transcription factors, phenotypic analysis
Location: 
Royal Victoria Hospital
Publications:
Soontorngun N, Larochelle M, Drouin S, Robert F, Turcotte B. Regulation of gluconeogenesis in Saccharomyces cerevisiae is mediated by activator and repressor functions of Rds2. Mol Cell Biol 27: 7895-7905; 2007.
MacPherson S, Larochelle M, Turcotte B. A fungal family of transcriptional regulators: the zinc cluster proteins. Microbiol Mol Biol 17: 58-604; 2006.
Larochelle M, Drouin S, Robert F, Turcotte B. Oxidative stress-activated zinc cluster protein Stb5 has dual activator/repressor functions required for pentose phosphate pathway regulation and NADPH production. Mol Cell Biol 26: 6690-6701; 2006.